Incubation:
Staining:Article Title: Vaccine elicitation of HIV broadly neutralizing antibodies from genome-edited B cells in non-human primates and derived lymphoid organoids.
Article Snippet: .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS. .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS.
Amplification:Article Title: Vaccine elicitation of HIV broadly neutralizing antibodies from genome-edited B cells in non-human primates and derived lymphoid organoids.
Article Snippet: .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS. .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS.
Magnetic Cell Separation:Article Title: Vaccine elicitation of HIV broadly neutralizing antibodies from genome-edited B cells in non-human primates and derived lymphoid organoids.
Article Snippet: .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS. .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS.
FACS:Article Title: Vaccine elicitation of HIV broadly neutralizing antibodies from genome-edited B cells in non-human primates and derived lymphoid organoids.
Article Snippet: .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS. .. Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Live/dead Blue Thermo Fisher L34961 1:1000 (stained separately) FcX NA 10 Gene Therapy Antibody Fluorophore Cat No Dilution factor or (μl/100ul stain) Biolegend 422301 BV Buffer NA BD 566385 10 CD3 BUV805 BD 742053 5 CD14 BUV805 BD 612903 2.5 CD20 BV605 Biolegend 302333 2.5 IgD AF488 Southern Biotech 2030-30 1.25 CD27 BV711 Biolegend 356429 10 CD21 BUV737 BD 612789 5 CD11c APC-R700 BD 566610 2.5 CD38 PerCP-Cy5.5 Caprico 100864 0.6 Lambda APC-vio770 Miltenyi 130- 122-223 1 Bcl-6 BUV496 BD 567753 5 Ki67 BV786 BD 563756 1.25 CD80 PE BD 557227 5 BiotinylatedKO11 BV421 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF555 Provided by Schief Lab (Scripps) 1 BiotinylatedeOD-GT8 AF647 Provided by Schief Lab (Scripps) 1 BiotinylatedMD39 AF555 Provided by Schief Lab (Scripps) 2 BiotinylatedMD39 AF647 Provided by Schief Lab (Scripps) 2 BiotinylatedAnti-P2A AF647 Millipore Sigma, MABS2005 1 BiotinylatedAnti-P2A AF555 Millipore Sigma, MABS2005 1 VRC01 cDNA amplification and gel B cells were enriched from lymphocytes by MACS or specific populations were sorted by FACS.
other:
|
Miltenyi Biotec is a verified supplier 
Miltenyi Biotec manufactures this product 